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CPT · 14% of the exam

Processing practice questions

10 free Processing questions for the Certified Phlebotomy Technician (CPT) exam, each with the correct answer and an explanation for every option.

What this domain covers

What happens after the draw: handling, transport, centrifuging and storing specimens correctly.

Exam weight
14%
On a real exam
≈ 17 questions
In our bank
81 questions

Topics that come up most

  • Specimen labelling
  • Transport temperatures and times
  • Centrifugation
  • Specimen rejection criteria
  • Storage requirements

How to study it

Know why specimens get rejected; many questions ask which handling error would invalidate a result.

Sample Processing questions

Try each one before opening the answer. Original questions written to the official exam objectives.

  1. Question 1

    How should tubes be held while waiting for transport or processing?

    • AUpright, stopper on top
    • BLying on their sides
    • CUpside down
    • DShaken every few minutes
    Show answer and explanation

    Answer: A) Upright, stopper on top

    • ACorrect. Keeping tubes upright helps clotting and reduces hemolysis and stopper contamination.
    • BIncorrect. This can affect clotting and contaminate stoppers.
    • CIncorrect. Blood would sit against the stopper.
    • DIncorrect. Shaking causes hemolysis.
  2. Question 2

    A test requires a frozen serum specimen, and the blood was collected in a plain red-top tube. Which processing sequence is correct?

    • AAllow it to clot completely, centrifuge, transfer the serum to a labeled tube, then freeze the serum
    • BFreeze the whole tube right away, then let the receiving lab thaw and centrifuge it on arrival
    • CCentrifuge it immediately after the draw, before it clots, then freeze the tube with serum on the cells
    • DRefrigerate the whole tube overnight so the clot retracts, then centrifuge and freeze the serum the next day
    Show answer and explanation

    Answer: A) Allow it to clot completely, centrifuge, transfer the serum to a labeled tube, then freeze the serum

    For frozen serum, let the specimen clot completely, centrifuge it promptly, move the serum to a labeled transfer tube, and then freeze it. Whole blood is never frozen, because that causes hemolysis.

    • ACorrect. Serum is only available after the blood has fully clotted and been centrifuged. CDC guidance is to transfer the separated serum to leakproof vials and freeze it right away.
    • BIncorrect. Freezing whole blood ruptures the red cells, causing hemolysis that contaminates the serum. Separate the serum before freezing.
    • CIncorrect. Spinning before the clot has formed doesn't give clean serum, and freezing serum on top of the cells causes hemolysis.
    • DIncorrect. Serum should be separated from the cells promptly. CDC guidance says to centrifuge within about 60 minutes of collection. Leaving serum on the cells for a long time can change analyte results.
  3. Question 3

    Physical, chemical and microscopic examination of urine is done in:

    • ABlood bank
    • BHistology
    • CUrinalysis
    • DSerology
    Show answer and explanation

    Answer: C) Urinalysis

    • AIncorrect. That handles transfusion testing.
    • BIncorrect. That handles tissues.
    • CCorrect. It's sometimes part of clinical microscopy.
    • DIncorrect. That handles antibody tests.
  4. Question 4

    Besides bilirubin, which analyte is commonly protected from light?

    • ASodium
    • BBeta-carotene
    • CGlucose
    • DPotassium
    Show answer and explanation

    Answer: B) Beta-carotene

    • AIncorrect. It isn't light-sensitive.
    • BCorrect. Vitamin A and some other vitamins are also light-sensitive.
    • CIncorrect. It isn't light-sensitive.
    • DIncorrect. It isn't light-sensitive.
  5. Question 5

    A glucose test arrives in a light-blue citrate tube instead of the required tube. Usually it is:

    • ARejected, and the correct tube recollected
    • BTested anyway
    • CPoured into a gray-top tube so the additive matches the order
    • DRelabeled as a coag test
    Show answer and explanation

    Answer: A) Rejected, and the correct tube recollected

    • ACorrect. The wrong additive can invalidate results.
    • BIncorrect. The wrong tube is rejected.
    • CIncorrect. Transferring doesn't fix it.
    • DIncorrect. That's improper.
  6. Question 6

    While performing a point-of-care glucose test, a phlebotomist gets a result within the facility's critical (panic) range. What should the phlebotomist do next?

    • AImmediately notify the patient's nurse or provider, following facility protocol
    • BEnter the value in the patient's chart for the provider's routine result review
    • CRepeat the test and report it only if the second result is also critical
    • DTell the patient the result and advise them to follow up with their provider
    Show answer and explanation

    Answer: A) Immediately notify the patient's nurse or provider, following facility protocol

    A critical (panic) value must be reported immediately to the nurse or provider responsible for the patient, following facility protocol. Routine charting, delays, or telling only the patient are not acceptable.

    • ACorrect. Critical values point to a possibly life-threatening condition. CLIA requires immediately alerting the person responsible for the patient, as the facility's critical-value protocol directs.
    • BIncorrect. Routine chart review could leave a life-threatening result unaddressed for hours. Critical values must be called to the responsible person right away.
    • CIncorrect. Some protocols allow a repeat check, but making notification depend on a second critical result delays care and could hide a real critical value.
    • DIncorrect. Telling only the patient doesn't get the result to the clinician who must act on it right away, and interpreting results is outside the phlebotomist's scope.
  7. Question 7

    Why is chain-of-custody documentation used for a legal blood alcohol specimen?

    • ATo keep the specimen cold
    • BTo make it process faster
    • CTo bill the patient's insurer
    • DTo prove who handled it
    Show answer and explanation

    Answer: D) To prove who handled it

    • AIncorrect. Storage is a separate requirement.
    • BIncorrect. The purpose is legal tracking, not speed.
    • CIncorrect. Billing is separate.
    • DCorrect. It records each person who handled the specimen so results hold up legally.
  8. Question 8

    A phlebotomist working in the lab is asked to phone a critical potassium result to the patient's nurse. Which step best confirms that the nurse received the result accurately?

    • AThe nurse repeats the patient's name and potassium value back to the caller
    • BThe nurse says the result is understood and will be passed on to the provider
    • CThe caller states the value twice, slowly, before ending the telephone call
    • DThe caller confirms the result has also been released to the electronic record
    Show answer and explanation

    Answer: A) The nurse repeats the patient's name and potassium value back to the caller

    When a critical value is reported by phone, the person receiving it reads back the patient identifier and result. The caller then documents the read-back to confirm the result was received accurately.

    • ACorrect. Read-back by the receiver closes the communication loop. It is a required part of critical value notification and is documented along with who gave and who received the result.
    • BIncorrect. A verbal acknowledgment does not show that the nurse heard the value and patient identity correctly. Only a read-back does that.
    • CIncorrect. Repeating the result from the caller's side does not check what the receiver actually heard and wrote down.
    • DIncorrect. Releasing the result electronically does not replace direct notification with read-back by the person receiving it.
  9. Question 9

    How should an ammonia specimen be transported to the lab?

    • AIn direct sunlight
    • BAt body temperature
    • CFrozen solid in the tube
    • DOn ice (chilled)
    Show answer and explanation

    Answer: D) On ice (chilled)

    • AIncorrect. Sunlight does not help preserve ammonia.
    • BIncorrect. Body-temperature transport is for specimens such as cold agglutinins.
    • CIncorrect. Whole blood is not frozen for transport.
    • DCorrect. Chilling slows changes that would falsely raise the ammonia level.
  10. Question 10

    A provider orders a rare, low-volume test that the hospital's own laboratory does not perform. How is this specimen usually handled?

    • AIt is sent to a reference laboratory, following that lab's collection, packaging and transport requirements
    • BIt is refrigerated in the hospital lab until enough orders for that test build up to run a batch onsite
    • CIt is run on the hospital's most similar in-house analyzer, and the result is reported under the ordered test
    • DIt is returned to the ordering provider's office so that staff there can arrange their own outside testing
    Show answer and explanation

    Answer: A) It is sent to a reference laboratory, following that lab's collection, packaging and transport requirements

    Reference laboratories do specialized or low-volume tests that the local lab doesn't offer. Specimens are processed and packaged to the reference lab's requirements before they are sent.

    • ACorrect. When the primary lab can't perform a test, the specimen is sent out to a reference lab. The phlebotomist must follow the reference lab's instructions for tube type, processing, temperature and packaging.
    • BIncorrect. The hospital lab doesn't offer the test, so holding the specimen won't get it tested. Delays can also make the specimen unusable.
    • CIncorrect. Reporting a different test's result as the ordered test is a serious error. Tests the lab can't perform are sent out.
    • DIncorrect. The laboratory handles send-out testing. It processes, packages and ships the specimen to the reference lab rather than returning it to the provider.

Processing: FAQs

How much of the CPT exam is Processing?

Processing is 14% of the CPT exam by the official exam guide's weighting, which on a 120-question form works out to roughly 17 questions. The real exam's exact split varies by form.

What does the Processing domain cover?

What happens after the draw: handling, transport, centrifuging and storing specimens correctly.

How should I study Processing for CPT?

Know why specimens get rejected; many questions ask which handling error would invalidate a result.

Where can I practise more Processing questions?

This page has 10 free Processing questions with answers. The ExamWize CPT question bank has 81 questions in this domain. Every practice exam result breaks your score down by domain, and Review Mode re-drills the questions you missed.

Get Processing exam-ready

The full CPT bank has 81 Processing questions. Timed practice exams match the real exam's length and domain weights, every result shows your score per domain, and Review Mode re-drills the questions you missed.

Not affiliated with NHA; questions are original, written from the official exam outline, and never copied from the real exam.