Safety and Compliance (26%)
- Bloodborne Pathogens Standard; Needlestick Safety and Prevention Act
- PPE and transmission-based precautions; hand hygiene
- Exposure response, sharps disposal, QC logs, HIPAA
Patient Preparation (20%)
- Two identifiers; match ID band and requisition
- Fasting and timed-draw requirements; consent
- Sites to avoid: IV arm, AV fistula, mastectomy side
Routine Blood Collections (28%)
- Order of draw: cultures → citrate → serum → heparin → EDTA → fluoride
- Tourniquet 3–4 inches above site, under 1 minute; bevel up, 15–30°
- Capillary: EDTA first; middle or ring finger; wipe the first drop
Special Collections and Processing (12% + 14%)
- Blood cultures, newborn screening, blood alcohol, tolerance tests
- Clot serum tubes before spinning; balance the centrifuge
- Light-protect bilirubin; chill ammonia; chain of custody
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